Life's Blood

ANTIBODY SCREENING WORK SHEET

When do you perform an antibody screening procedure?

 

 

What phases must be included in an antibody screening? 

 

Below is a 3-cell panel used for antibody screening (gamma biologicals, inc., Lot No. 082656 that outdates October 1, 2003)

 

Rh

MNSs

 P Lewis

Kell

Duffy

Kidd

Sex-linked

 37oC AGT CCC
  D C E c e M N S s P1 Lea Leb K k Kpa Jsa Fya Fyb Jka Jkb Xga Sex
1 + + 0 0 + + 0 + + + + 0 0 + 0 0 + 0 + + + /      
2 + 0 + + 0 + 0 + + + 0 + 0 + 0 0 + + + 0 + /      
3 0 0 0 + + + + 0 + + 0 + + + 0 0 0 + 0 + + /      

Since the Trio Cells 1, 2, and 3 should not detect anti-A or anti-B, what ABO type must they be?


In this antibody screening cell panel, the Rh antigens are all homozygous.  In the rest of the  screening cell panel, which antigens are heterozygous (both alleles are positive) in:
Cell 1

Cell 2

Cell 3

If the patient is A positive, and the following results were obtained 

 

Rh

MNSs

 P Lewis

Kell

Duffy

Kidd

Sex-linked

 37oC AGT CCC
  D C E c e M N S s P1 Lea Leb K k Kpa Jsa Fya Fyb Jka Jkb Xga Sex
1 + + 0 0 + + 0 + + + + 0 0 + 0 0 + 0 + + + / 0 0 2+
2 + 0 + + 0 + 0 + + + 0 + 0 + 0 0 + + + 0 + / 0 0 2+
3 0 0 0 + + + + 0 + + 0 + + + 0 0 0 + 0 + + / 0 3+  

Which two antibodies could show this pattern?

Which of the two is most likely since it only showed up at AGT?

What is the usual temperature of reactions for the antibody to the other antigen?

Why don't we perform the antibody screening procedure at that temperature?

Why were the Coombs Control Check Cells on performed on cells 1 and 2?

If the patient below  is woman, with 3 previous pregnancies, types O negative, and has received Rho Immune Globulin during and after each pregnancy, what antibody is probably showing up.

 

Rh

MNSs

 P Lewis

Kell

Duffy

Kidd

Sex-linked

 37oC AGT CCC
  D C E c e M N S s P1 Lea Leb K k Kpa Jsa Fya Fyb Jka Jkb Xga Sex
1 +/ +/ 0 0 +/ +/ 0 + + + + 0 0 +/ 0 0 +/ 0 + + + / 0 0 2+
2 + 0 + + 0 + 0 + + + 0 + 0 + 0 0 + + + 0 + / 1+ 3+  
3 0 0 0 + + + + 0 + + 0 + + + 0 0 0 + 0 + + / 0 0 2+

Go to the first negative reaction with a negative result and cross out any homozygous antigens (This is done for you in red.) These are ruled out as possible antibodies since the cells are strong acting and this panel shows no reactions. 

What  is different about Lewis inheritance that does not allow us to cross out those antigens?

 

What antibodies are still possible after doing the first cross-out of homozygous cells where you have a negative reaction?

What is the likelihood that this antibody is anti-Kpa or anti-Jsa?

Now perform a cross-out of homozygous cells for cell 3 since that also gave a negative reaction.  What additional antibodies were you able to eliminate when you performed this cross out?

 

What antibody is most likely?

 

What other antibodies will you need to eliminate?

 
 

Clinical Microbiology Syllabus