Laboratory 5:  Urine Culture Unknown and Sensitivity 

Activities:

• NCCLS "Disk Susceptibility Testing: Step by Step" Video (LECTURE)
• Demo Susceptibility Testing 
• Read E.coli Kirby-Bauer Susceptibility Plate with instructor
• Compare E.coli and Contaminated Urine Culture Plates with instructor
• Set Up Urine Unknown (we will wait and do this next week on Weds.

• Demo for ONPG and PYR/Esculin Rapid Tests

• API Set-up Demo Station
• Complete and
• Review for Practical  
• Study Guide for Skin and Soft Tissue This should be completed by Wed. October 4, 2006 and can be found under assignments in Blackboard
• Unit 1 Lecture Exam is scheduled for Thursday, September 28, 2006 at 0730. The practical exam is scheduled for the full three hours on Wednesday October 4, 2006.

Read E.coli Kirby-Bauer Susceptibility Plate with Instructor

Materials Needed:

1. Sensitivity Plate

2. Ruler with millimeter markings

3. NCCLS Disk Susceptibility Testing: Step by Step

4. NCCLS-M100 "Table 2. Zone Diameter Interpretative Standards and Equivalent Minimum Inhibitory Concentration (MIC) Breakpoints for Organisms Other than Haemophilus and Neisseria gonorrhoeae"

5. Kirby Bauer Susceptibility Report Form (Green Sheet with list of antimicrobics)

6. Enter the susceptibility report as you would to the physician in the Susceptibility Report Form

Compare E.coli and Contaminated Urine Culture Plates with Instructor

A station is set-up with culture plates for a probable infection of E.coli and other urine cultures that contain skin and vaginal contaminants. Answer the questions on the Urine Culture Comparison Worksheet for this station.

Top.

Demo for ONPG and PYR/Esculin Rapid Tests with Instructor

Materials Needed:

1. Laboratory Manual p. 25 and product insert for ONPG disks.

2. ONPG and PYR/Esculin Disks from refrigerator

3. Serratia and Proteus culture plates to perform ONPG test

4. Streptococcus pyogenes, Streptococcus bovis and Enterococcus faecalis culture plates to perform the PYR/Esculin tests

5. Tube of sterile saline from refrigerator

6. Sterile plastic tubes in cupboard under centrifuge.

API Set-up Demo

UNIT 1 LAB PRACTICAL COMPONENTS

• Gram Stain Quality Control Slide

• Evaluate plate streaked the previous day and Gram Stain colony or colonies and describe the colonial morphology of each

• Evaluate types of hemolysis

• Identify the likely Gram stain reaction due to growth characteristics on plates such as CNA, MAC, EMB, TERG

• Organisms growing only on chocolate, Gram stain reaction and morphology and their atmospheric preferences

• Performance of wet prep (yeast or bacteria), catalase, oxidase, spot indole, and coagulase.

• Know the reagent names for each of the rapid tests listed above and also the bile solubility test

• Based on Gram stain reaction and morphology, determine whether to do oxidase or catalase.

• Organisms that you are trying to isolate if you use MTM, SXT agar

• Recognize the colonial appearance of Proteus, Klebsiella, E.coli, Pseudomonas

• Rank order what specimens would be cultured first, second, third, etc.

• Read Gram Stains for Gram stain reaction (positive or negative) and morphology (cocci, rods, yeast) and name at least one organism it could be

• Evaluate 2 sputum specimens for quality on low power

• A couple of safety questions

Unit 1 Practical Review