Worksheet relating to Media Charts I and II and Laboratory Manual Information

1. If you have colonies growing on only Chocolate and Modified Thayer Martin and not on any the other plates, what organism(s) would you suspect?

2. What culture types might have this(ese) organism(s)?

3. Which types of specimens are more likely to contain Enterobacteriaceae organisms according to these 2 charts?
   

4. What organisms are we generally not expecting to find in wound cultures?

5. Give three examples of cultures that would need a special request before we would add alternative media such as MTM.
   

6. Consulting the Remel media sheets indicate the expected results for each of the organisms listed for the specific media:

   Streptococcus pyogenes	TSA Sheep Blood Agar
   	CNA agar
   	SXT agar
   	Bile esculin agar
   	Tergitol 7
   Staphylococcus aureus	TSA Sheep Blood Agar
   	CNA agar
   	SXT agar
   	Modified Thayer Martin agar
   	MacConkey agar
   Enterococcus faecalis	CNA agar
   	SXT agar
   	Bile esculin agar
   Haemophilus influenzae	Chocolate agar
   	Modified Thayer Martin agar
   Neisseria gonorrhoeae	Chocolate agar
   	Modified Thayer Martin agar
   S. pneumoniae	TSA Sheep Blood Agar

7. Why do you think some of these organisms are *NCCLS recommended organisms and others are designated as to be used only by commercial manufacturers and others have no designation under Quality Control even though their reactions are listed?

Name: _______________________  Date: _________

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