MADISON AREA TECHNICAL COLLEGE
CLINICAL LABORATORY TECHNICIAN PROGRAM

Week 2 Laboratory 2

Name: _____________________________________        Date: _____________________

Quality Control Slide: Prepare a gram stain slide of control organisms by suspending the organism in a drop of saline, letting it dry, and gram staining it.  Unknowns: When your control slide is checked off you may gram stain and report organisms 1-8. You may take a stab at the organism by looking them up in your text by culture source and gram stain morphology. Look at the slide under oil immersion; do not worry about quantification at this point of the semester.

Organism Gram Reaction & Morphology Does this slide meet the gram stain standard? S. aureus should be a gram+ cocci (GPC) in clusters. E. coli is a fat gram negative rod. (GNR) SHOW ME!
Staphylococcus aureus    
E.coli

 

   

Slide #

Culture Source

Gram Stain & Morphology

Possible Organism?

1

Isolate from Wound Culture

2

Isolate from Wound Culture (possible skin contaminant)

3

Isolate of a possible contaminant

4

Isolate from a Neonatal CSF

5

Isolate from a Vaginal Culture

6

Isolate from a Urine Culture

7

Isolate from a Burn Infection

8

Isolate from an Urethral Discharge

 

Clinical Microbiology Syllabus