Which organism(s) produce "sulfa granules" in the exudate
from the infection? ANSWER: Nocardia
and Actinomyces
If you gram stain the yellow flecks of "sulfa granules"
what would be the gram stain reaction and morphology of the organism(s)?
ANSWER:Gram positive rods that are long,
filamentous and branching
Which of the organisms is aerobic and which is anaerobic? ANSWER:
Nocardia is aerobic and Actinomyces are anaerobic
The lower picture is a Gram stain and the top picture is a modified
acid stain of the same organism. What branching gram positive rod
is acid fast? ANSWER: Nocardia
What would the colonial appearance of this organism? ANSWER:
glaborous or like molar teeth, some also are describe as bread crumb
like
What type of specimens might contain this organism? ANSWER: subcutaneous
abscesses, sputum, jaw infections
What is the name of the black lesion by this man's ear? ANSWER: black
eschar
What organism causes this lesion? ANSWER: Bacillus
anthracis
Is this organism beta hemolytic on blood agar? ANSWER: No
What is the Gram stain morphology of the organism? ANSWER: Gram
positive rods with spores
What is the lecithinase result on Egg Yolk agar? ANSWER:Positive
If you had a nonhemolytic colony on blood that was motile, could you
rule out this organism? ANSWER:Yes since B.anthracis
is nonmotile
This is Tinsdale Agar that is used for what organism? ANSWER: Corynebacterium
diphtheriae
What other selective media can be used for this organism? ANSWER: Loeffler's,
Cystine tellurite etc.
What is the name of the test used to determine if this organism
produces the toxin that causes the disease? ANSWER: Elek
plate with a anti-toxin strip that reacts with the toxin.
(Precipitation antigen/antibody test)
Where might you find other organisms of this genus? ANSWER:
What disease does this Gram-positive organism cause? ANSWER: erysipliod
Who is most likely to get this infection and where? ANSWER:
Hog farmers
What is the catalase reaction for this organism? ANSWER:
negative
This Gram-positive rod is faintly beta hemolytic and blood and may
look like Group B streptococci; what is it? ANSWER: Listeria
monocytogenes
What would be the appearance of this organism on Bile Esculin agar? ANSWER: black
colony with a black halo
What would be the appearance of the motility in a wet prep? ANSWER: Tumbline
What would be the CAMP test result? ANSWER: Positive
What type of food products may result in food poisoning due to this
organism? ANSWER: Mushrooms, milk
products, deli meats
Are either Group B or Group D streptococci motile? ANSWER: No
streps are motile
